Did you try another set of speakers on the suspect mobo? Or a set of headphones? I'm assuming you're using the output jack on the rear I/O panel.
Have you checked all your Volume Control settings? Sounds and Audio Output Device settings? Output levels? Muted output checkbox anywhere?
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Frankenbox III:
CPU: AMD Athlon 64 X2 4200+ *** MOBO: Biostar K8M800 Micro AM2 *** RAM: 2x1GB Kingston DDR2 667
HDD: WD 80GB Master, Seagate 320GB Slave *** OPT: two Pioneer DVR-115DBK DVD burners
PSU: Antec Basiq 500W *** GPU: PNY GeForce FX5200 256MB AGP 8x *** MON: ViewSonic LCD VA721
CASE: EvTek minitower *** SND: Diamond Xtreme 5.1 *** OS: XP Home SP2 *** ROUTER: Linksys BEFSR41
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