Would this work on my desktop?

Yes it will work, but you will need a floppy cable (FDD Cable), and a floppy power connection.
 
yep, just unplug the ribbon cable from the back of the floppy drive, plug it into the Card reader, then take the power connection from the floppy, then put it into the card reader, mount the Card reader in your case, then your all set
 
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